Jump to main content
Jump to site search

Issue 48, 2011
Previous Article Next Article

Scanning near-field IR microscopy of proteins in lipid bilayers

Author affiliations

Abstract

We use infrared near-field microscopy to chemically map the morphology of biological matrices. The investigated sample is built up from surface-tethered membrane proteins (cytochrome c oxidase) reconstituted in a lipid bilayer. We have carried out infrared near-field measurements in the frequency range between 1600 and 1800 cm−1. By simultaneously recording the topography and chemical fingerprint of the protein-tethered lipid bilayer with a lateral resolution of 80 nm × 80 nm, we were able to probe locally the chemical signature of this membrane and to provide a local map of its surface morphology.

Graphical abstract: Scanning near-field IR microscopy of proteins in lipid bilayers

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 11 May 2011, accepted on 05 Oct 2011 and first published on 02 Nov 2011


Article type: Paper
DOI: 10.1039/C1CP21512D
Citation: Phys. Chem. Chem. Phys., 2011,13, 21432-21436
  •   Request permissions

    Scanning near-field IR microscopy of proteins in lipid bilayers

    F. Ballout, H. Krassen, I. Kopf, K. Ataka, E. Bründermann, J. Heberle and M. Havenith, Phys. Chem. Chem. Phys., 2011, 13, 21432
    DOI: 10.1039/C1CP21512D

Search articles by author