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Issue 28, 2011
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Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions

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Abstract

Stacking interactions between organic fluorophores can cause formation of non-fluorescent H-dimers. Dimer formation and dissociation of two fluorophores site-specifically incorporated in a biomolecule result in fluorescence intermittency that can report on conformational dynamics. We characterize intramolecular dimerization of two oxazine fluorophores MR121 attached to an unstructured polypeptide. Formation of stable non-fluorescent complexes with nano- to microsecond lifetimes is a prerequisite for analysing the intermittent fluorescence emission by fluorescence correlation spectroscopy and extracting relaxation time constants on nano- to millisecond time scales. Destabilization of the generally very stable homodimers by chemical denaturation reduces the lifetime of H-dimers. We demonstrate that H-dimer formation of an oxazine fluorophore reports on end-to-end contact rates in unstructured glycineserine polypeptides under denaturing conditions.

Graphical abstract: Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions

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Publication details

The article was received on 08 Apr 2011, accepted on 24 May 2011 and first published on 20 Jun 2011


Article type: Paper
DOI: 10.1039/C1CP21111K
Citation: Phys. Chem. Chem. Phys., 2011,13, 12874-12882
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    Dimer formation of organic fluorophores reports on biomolecular dynamics under denaturing conditions

    S. Bollmann, M. Löllmann, M. Sauer and S. Doose, Phys. Chem. Chem. Phys., 2011, 13, 12874
    DOI: 10.1039/C1CP21111K

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