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Issue 16, 2011
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Influence of percentage of guanine molecules, OH radicals, UV irradiation and temperature on electrooxidation of short synthetic oligonucleotides

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Abstract

The electrooxidation of short synthetic 20-nucleotides DNA sequences with various amount of guanine molecules has been studied in a wide temperature range by square wave voltammetry and the results were compared with UV–vis and CD spectra. A twofold increase of dsDNA voltammetric peak, related to an increase in the number of electrons transfered in the guanine electrooxidation process was found to begin at a temperature lower by circa 20 °C compared to the well known increase of the dsDNA absorbance upon denaturation. Since the dsDNA voltammetric peaks are related directly to the electrooxidation of guanine and adenine, early conformational changes in dsDNA are responsible for this effect. An increase in percentage of guanine in the DNA chains caused a delay in the conformational, predenaturation changes. An exception to this behavior was found for polyguanine (100% guanine). Interestingly, two distinct ranges of change in ellipticity in the CD spectra correlate well with the changes obtained by voltammetry. We have also checked the influence of OH radicals and UV irradiation on the dsDNA oxidation.

Graphical abstract: Influence of percentage of guanine molecules, OH radicals, UV irradiation and temperature on electrooxidation of short synthetic oligonucleotides

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Publication details

The article was received on 25 Nov 2010, accepted on 25 Feb 2011 and first published on 21 Mar 2011


Article type: Paper
DOI: 10.1039/C0CP02667K
Citation: Phys. Chem. Chem. Phys., 2011,13, 7500-7507
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    Influence of percentage of guanine molecules, OH radicals, UV irradiation and temperature on electrooxidation of short synthetic oligonucleotides

    A. M. Nowicka, E. Zabost, E. Gilant and Z. Stojek, Phys. Chem. Chem. Phys., 2011, 13, 7500
    DOI: 10.1039/C0CP02667K

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