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Issue 15, 2011
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Protic equilibria as the key factor of quercetin emission in solution. Relevance to biochemical and analytical studies

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Abstract

A detailed spectrofluorimetric study on quercetin in aqueous solution proves that its anionic forms are responsible for a strong fluorescence enhancement observed at pH > 6. Anion fluorescence is also observed in organic solvents with strong hydrogen bond acceptor properties. The results provide a new interpretation of biophysical and analytical literature data where the fluorescence of the anionic forms of quercetin has never been explicitly taken into account. In particular, comparison with published binding studies strongly suggests that quercetin deprotonation and consequent fluorescence enhancement takes place when the flavonoid binds to several biological macromolecules. This observation can be very helpful in the understanding at a molecular level of the interaction between quercetin and the biomolecule.

Graphical abstract: Protic equilibria as the key factor of quercetin emission in solution. Relevance to biochemical and analytical studies

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Publication details

The article was received on 27 May 2010, accepted on 11 Feb 2011 and first published on 09 Mar 2011


Article type: Paper
DOI: 10.1039/C0CP00714E
Citation: Phys. Chem. Chem. Phys., 2011,13, 6858-6864
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    Protic equilibria as the key factor of quercetin emission in solution. Relevance to biochemical and analytical studies

    A. Mezzetti, S. Protti, C. Lapouge and J. Cornard, Phys. Chem. Chem. Phys., 2011, 13, 6858
    DOI: 10.1039/C0CP00714E

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