Structural control of surface layer proteins at electrified interfaces investigated by in situFourier transform infrared spectroscopy

Abstract

In situ Fourier Transform Infrared (FTIR) Spectroscopy complemented by Electrochemical Quartz Microbalance (EQMB) investigations allowed a detailed insight into the influence of the electrode potential on competing adsorption processes and bonding mechanisms of buffer ions and S-layer protein molecules of Lysinibacillus sphaericusCCM2177 at an electrified liquid/gold interface. The S-layer proteins adsorb on gold polarized positively of the point of zero charge by displacing perchlorate anions in the Helmholtz plane by their carboxylate groups. This is indicated by an increase of the peptide and carboxylate infrared absorption signals accompanied by a decrease of the perchlorate signal. S-layers interlinked laterally with Ca²⁺ ions, positive of the point of zero charge, resulted in the formation of a crystalline layer participating in the Helmholtz layer. In contrast to the absence of the Ca²⁺-linkers, S-layers remain structurally intact also in the negative polarization domain where the Helmholtz layer is solely sustained by mainly solvated cations without participation of the negatively charged protein carboxylate functions.