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Issue 45, 2011
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Unexpected structure of a C. difficiletoxin A ligand necessitates an annotation correction in a popular screening library

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Abstract

The structure of the pentasaccharide S259-1 in the Consortium for Functional Glycomics was investigated using a variety of techniques. Surprisingly, the structure differs from the structure assumed from the previously established specificity of the human fucosyltransferase FUT-III used in the last step of chemoenzymatic synthesis. When presented with a tetrasaccharide substrate containing both type I and type II disaccharide moieties, the enzyme generates a pentasaccharide in which the type II moiety is preferentially fucosylated. The unexpected product generated by FUT-III in this case highlights the importance of performing detailed structural analysis on products generated by enzymes.

Graphical abstract: Unexpected structure of a C. difficiletoxin A ligand necessitates an annotation correction in a popular screening library

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Publication details

The article was received on 27 Aug 2011, accepted on 27 Sep 2011 and first published on 21 Oct 2011


Article type: Communication
DOI: 10.1039/C1CC15344G
Citation: Chem. Commun., 2011,47, 12397-12399
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    Unexpected structure of a C. difficiletoxin A ligand necessitates an annotation correction in a popular screening library

    P. Zhang, N. Razi, L. Eugenio, M. Fentabil, E. N. Kitova, J. S. Klassen, D. R. Bundle, K. K.-S. Ng and C. Ling, Chem. Commun., 2011, 47, 12397
    DOI: 10.1039/C1CC15344G

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