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Issue 18, 2011
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LC-MS based quantification of 2′-ribosylated nucleosides Ar(p) and Gr(p) in tRNA

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Abstract

RNA nucleosides are often naturally modified into complex non-canonical structures with key biological functions. Here we report LC-MS quantification of the Ar(p) and Gr(p) 2′-ribosylated nucleosides in tRNA using deuterium labelled standards, and the first detection of Gr(p) in complex fungi.

Graphical abstract: LC-MS based quantification of 2′-ribosylated nucleosides Ar(p) and Gr(p) in tRNA

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Publication details

The article was received on 21 Feb 2011, accepted on 13 Mar 2011 and first published on 29 Mar 2011


Article type: Communication
DOI: 10.1039/C1CC11011J
Citation: Chem. Commun., 2011,47, 5196-5198
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    LC-MS based quantification of 2′-ribosylated nucleosides Ar(p) and Gr(p) in tRNA

    D. Pearson, A. Hienzsch, M. Wagner, D. Globisch, V. Reiter, D. Özden and T. Carell, Chem. Commun., 2011, 47, 5196
    DOI: 10.1039/C1CC11011J

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