Fluorescence detection of coralyne and polyadenylation reaction using an oligonucleotide-based fluorogenic probe

Abstract

This study describes the development of a simple, sensitive, and selective-detection system for coralyne and polyadenylation reaction based on the fact that coralyne induces a conformational change of the polyadenosine [poly(A)] oligonucleotide through A–coralyne–A coordination, thereby enhancing the fluorescence of SYBR Green I.