Issue 16, 2011
From the journal:

Chemical Communications

Ultrasensitive endonuclease activity and inhibition detection using gold nanoparticle-enhanced fluorescence polarization†

Yong Huang,ab   Shulin Zhao,*a   Zhen-Feng Chen,a   Yan-Cheng Liua  and  Hong Liang*a  

* Corresponding authors

a Ministry of Education Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China
E-mail: zhaoshulin001@163.com, hliang@gxnu.edu.cn
Fax: (+86) 773-583-2294
Tel: (+86) 773 5856104

b College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, China

Abstract

We have developed a highly sensitive and selective fluorescence polarization assay method based on the specificity of the DNA cleavage reaction with the enhancement of gold nanoparticles (AuNPs) for assaying endonuclease activity and inhibition. This assay can detect EcoRI endonuclease down to 5.0 × 10−4 U mL−1 with a detection range from 5.0 × 10−4 to 10 U mL−1.

Graphical abstract: Ultrasensitive endonuclease activity and inhibition detection using gold nanoparticle-enhanced fluorescence polarization

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1CC10325C
Article type
Communication
Submitted
18 Jan 2011
Accepted
24 Feb 2011
First published
11 Mar 2011

Chem. Commun., 2011,47, 4763-4765

Permissions

Request permissions

Ultrasensitive endonuclease activity and inhibition detection using gold nanoparticle-enhanced fluorescence polarization

Y. Huang, S. Zhao, Z. Chen, Y. Liu and H. Liang, Chem. Commun., 2011, 47, 4763 DOI: 10.1039/C1CC10325C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements