Issue 5, 2011

Tetracyanoquinodimethanenanoparticles as an effective sensing platform for fluorescent nucleic acid detection

Abstract

In this communication, we report on the use of tetracyanoquinodimethane nanoparticles (TNs) as an effective fluorescent sensing platform for nucleic acid detection for the first time. The general concept used in this approach is based on adsorption of fluorescently labeled single-stranded DNA (ssDNA) probe by TN, due to the strong π–π stacking between unpaired DNA bases and TN. As a result, the fluorophor is brought into close proximity of TN, leading to substantial fluorescence quenching via photoinduced electron transfer between fluorescent dye and TN. Upon presence of the target ssDNA, specific hybridization with the target takes place to form a double-stranded DNA (dsDNA). The helix cannot be adsorbed by TN due to its rigid conformation and the absence of unpaired DNA bases. Thus, the fluorophor is seperated from TN accompanied by fluorescence recovery. This fluorescence enhancement signals completion of the assay. It also suggests that this sensing platform can well differentiate perfect complementary and mismatched sequences. A detection limit as low as 1.5 nM was obtained.

Graphical abstract: Tetracyanoquinodimethane nanoparticles as an effective sensing platform for fluorescent nucleic acid detection

Article information

Article type
Communication
Submitted
08 Dec 2010
Accepted
18 Mar 2011
First published
04 Apr 2011

Anal. Methods, 2011,3, 1051-1055

Tetracyanoquinodimethane nanoparticles as an effective sensing platform for fluorescent nucleic acid detection

H. Li, L. Wang, J. Zhai, Y. Luo, Y. Zhang, J. Tian and X. Sun, Anal. Methods, 2011, 3, 1051 DOI: 10.1039/C0AY00746C

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