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Issue 22, 2011
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Development of an integrated metabolomic profiling approach for infectious diseases research

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Abstract

Metabolomic profiling offers direct insights into the chemical environment and metabolic pathway activities at sites of human disease. During infection, this environment may receive important contributions from both host and pathogen. Here we apply an untargeted metabolomics approach to identify compounds associated with an E. coli urinary tract infection population. Correlative and structural data from minimally processed samples were obtained using an optimized LC-MS platform capable of resolving ∼2300 molecular features. Principal component analysis readily distinguished patient groups and multiple supervised chemometric analyses resolved robust metabolomic shifts between groups. These analyses revealed nine compounds whose provisional structures suggest candidate infection-associated endocrine, catabolic, and lipid pathways. Several of these metabolite signatures may derive from microbial processing of host metabolites. Overall, this study highlights the ability of metabolomic approaches to directly identify compounds encountered by, and produced from, bacterial pathogens within human hosts.

Graphical abstract: Development of an integrated metabolomic profiling approach for infectious diseases research

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Publication details

The article was received on 13 Jul 2011, accepted on 18 Aug 2011 and first published on 16 Sep 2011


Article type: Paper
DOI: 10.1039/C1AN15590C
Citation: Analyst, 2011,136, 4752-4763
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    Development of an integrated metabolomic profiling approach for infectious diseases research

    H. Lv, C. S. Hung, K. S. Chaturvedi, T. M. Hooton and J. P. Henderson, Analyst, 2011, 136, 4752
    DOI: 10.1039/C1AN15590C

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