Issue 20, 2011

Design and evaluation of synthetic silica-based monolithic materials in shrinkable tube for efficient proteinextraction

Abstract

Sample pretreatment is a required step in proteomics in order to remove interferences and preconcentrate the samples. Much research in recent years has focused on porous monolithic materials since they are highly permeable to liquid flow and show high mass transport compared with more common packed beds. These features are due to the micro-structure within the monolithic silica column which contains both macropores that reduce the back pressure, and mesopores that give good interaction with analytes. The aim of this work was to fabricate a continuous porous silica monolithic rod inside a heat shrinkable tube and to compare this with the same material whose surface has been modified with a C18 phase, in order to use them for preconcentration/extraction of proteins. The performance of the silica-based monolithic rod was evaluated using eight proteins; insulin, cytochrome C, lysozyme, myoglobin, β-lactoglobulin, ovalbumin, hemoglobin, and bovine serum albumin at a concentration of 60 μM. The results show that recovery of the proteins was achieved by both columns with variable yields; however, the C18 modified silica monolith gave higher recoveries (92.7 to 109.7%) than the non-modified silica monolith (25.5 to 97.9%). Both silica monoliths can be used with very low back pressure indicating a promising approach for future fabrication of the silica monolith inside a microfluidic device for the extraction of proteins from biological media.

Graphical abstract: Design and evaluation of synthetic silica-based monolithic materials in shrinkable tube for efficient protein extraction

Article information

Article type
Paper
Submitted
31 May 2011
Accepted
04 Aug 2011
First published
24 Aug 2011

Analyst, 2011,136, 4321-4327

Design and evaluation of synthetic silica-based monolithic materials in shrinkable tube for efficient protein extraction

E. Alzahrani and K. Welham, Analyst, 2011, 136, 4321 DOI: 10.1039/C1AN15447H

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