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Issue 22, 2011
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Electrochemical identification of artificial oligonucleotides related to bovine species. Potential for identification of species based on mismatches in the mitochondrial cytochrome C1 oxidase gene

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Abstract

Our studies show that electrochemical impedance spectroscopy (EIS) and scanning electrochemical microscopy (SECM) of films of ds-DNA on gold allow us to distinguish between mitochondrial DNA fragments of the cytochrome c1 oxidase (mt-Cox1) of three related species of the subfamily ‘Bovinae’ (Bos taurus, Bison bison, and Bison bonasus). In EIS, a perfectly matched DNA gives rise to a considerably larger charge transfer resistance Rct compared to mismatched pairings. Differences in charge transfer resistance, ΔRct, before and after the addition of Zn2+ ions provide an additional tool for identification. In addition, all ds-DNA films were studied by SECM and their kinetic parameters were determined. Perfectly matched ds-DNAs are readily distinguished from mismatched duplexes by their lower rate constants. Our system can be used multiple times by dehybridization and rehybridization of capture strands up to the 250 pmole level.

Graphical abstract: Electrochemical identification of artificial oligonucleotides related to bovine species. Potential for identification of species based on mismatches in the mitochondrial cytochrome C1 oxidase gene

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Publication details

The article was received on 19 May 2011, accepted on 26 Jul 2011 and first published on 17 Aug 2011


Article type: Paper
DOI: 10.1039/C1AN15414A
Citation: Analyst, 2011,136, 4724-4731
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    Electrochemical identification of artificial oligonucleotides related to bovine species. Potential for identification of species based on mismatches in the mitochondrial cytochrome C1 oxidase gene

    M. H. Shamsi and H. Kraatz, Analyst, 2011, 136, 4724
    DOI: 10.1039/C1AN15414A

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