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Issue 20, 2011
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Production of rapidly reversible antibody and its performance characterization as binder for continuous glucose monitoring

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Abstract

To effectively control diabetes, a method to reliably measure glucose fluctuations in the body over given time periods needs to be developed. Current glucose monitoring systems depend on the substrate decomposition by an enzyme to detect the product; however, the enzyme activity significantly decays over time, which complicates analysis. In this study, we investigated an alternative method of glucose analysis based on antigen–antibody binding, which may be active over an extended period of time. To produce monoclonal antibodies, mice were immunized with molecular weight (MW) 10K dextran chemically conjugated with keyhole limpet hemocyanin. Since dextran contains glucose molecules polymerized via a 1,6-linkage, the produced antibodies had a binding selectivity that could discriminate biological glucose compounds with a 1,4-linkage. Three antibody clones with different affinities were screened using the MW 1K dextran–bovine serum albumin conjugates as the capture ligand. Among the antibodies tested, the antibody clone Glu 26 had the lowest affinity (KA = 3.56 × 106 M−1) and the most rapid dissociation (kd = 1.17 × 10−2 s−1) with the polysaccharide immobilized on the solid surfaces. When glucose was added to the medium, the sensor signal was inversely proportional to the glucose concentration in a range between 10 and 1000 mg dL−1, which covered the clinical range. Under the optimal conditions, the response time was about 3 min for association and 8 min for dissociation based on a 95% recovery of the final equilibrium.

Graphical abstract: Production of rapidly reversible antibody and its performance characterization as binder for continuous glucose monitoring

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Publication details

The article was received on 21 Apr 2011, accepted on 02 Aug 2011 and first published on 31 Aug 2011


Article type: Paper
DOI: 10.1039/C1AN15338B
Citation: Analyst, 2011,136, 4268-4276
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    Production of rapidly reversible antibody and its performance characterization as binder for continuous glucose monitoring

    S. Paek, I. Cho, S. Seo, D. Kim and S. Paek, Analyst, 2011, 136, 4268
    DOI: 10.1039/C1AN15338B

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