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Issue 18, 2011
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Molecularly imprinted monolith coupled on-line with high performance liquid chromatography for simultaneous quantitative determination of cyromazine and melamine

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Abstract

We report a novel method for simultaneous determination of cyromazine and melamine based on a molecularly imprinted monolith on-line coupled with high performance liquid chromatography (HPLC). The imprinted monolith was prepared by in situpolymerization using 2,4-diamino-6-undecyl-1,3,5-triazine (DAUTA) as a mimic template. Due to the better solubility of DAUTA in chloroform, hydrogen bonds were effectively developed between the template and the functional monomer and resulted in the formation of highly specific cavities in the obtained imprinted monolith. With methanol as the loading solvent, cyromazine and melamine were both selectively retained by the obtained imprinted monolith, while the nonspecific adsorption on the non-imprinted monolith was negligible. The imprinted monolithic column was on-line coupled with HPLC for purification and concentration of the two analytes from milk samples. To minimize the peak broadening during the on-line transfer of the analytes from the imprinted monolith to the following analytical column, a successive desorption program was developed for the elution step, which enabled on-line stacking of the target compounds before being analyzed by HPLC. Low detection limits of 0.12 μg mL−1 for melamine and 0.05 μg mL−1 for cyromazine were achieved with only 0.3 mL of milk sample and a low sensitivity HPLC-UVD instrument. The method may be further extended to detect other analytes of interest in a large variety of samples.

Graphical abstract: Molecularly imprinted monolith coupled on-line with high performance liquid chromatography for simultaneous quantitative determination of cyromazine and melamine

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Publication details

The article was received on 31 Jan 2011, accepted on 09 Jun 2011 and first published on 25 Jul 2011


Article type: Paper
DOI: 10.1039/C1AN15086C
Citation: Analyst, 2011,136, 3672-3679
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    Molecularly imprinted monolith coupled on-line with high performance liquid chromatography for simultaneous quantitative determination of cyromazine and melamine

    S. Wang, D. Li, Z. Hua and M. Zhao, Analyst, 2011, 136, 3672
    DOI: 10.1039/C1AN15086C

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