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Issue 16, 2011
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Infrared spectromicroscopy of biochemistry in functional single cells

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Abstract

Over the years Fourier-Transform Infrared (FTIR) spectroscopy has been widely employed in the structural and functional characterization of biomolecules. The introduction of infrared (IR) microscopes and of synchrotron light sources has created expectations that FTIR could become a generally viable technique to study both structure and reactivity in vivo, inside single cells, by performing measurements that up to a few years ago were the preserve of in vitro experiments on purified macromolecules. In this review we present the state-of-the-art in the application of FTIR spectromicroscopy as a technique for the study of structure and dynamics in single cells, we discuss the performance requirements for this application and review developments in sample handling methods.

Graphical abstract: Infrared spectromicroscopy of biochemistry in functional single cells

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Publication details

The article was received on 23 Jan 2011, accepted on 03 May 2011 and first published on 15 Jun 2011


Article type: Critical Review
DOI: 10.1039/C1AN15060J
Citation: Analyst, 2011,136, 3219-3232
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    Infrared spectromicroscopy of biochemistry in functional single cells

    L. Quaroni and T. Zlateva, Analyst, 2011, 136, 3219
    DOI: 10.1039/C1AN15060J

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