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Issue 17, 2011
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Single particle tracking as a method to resolve differences in highly colocalized proteins

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Abstract

Single particle tracking fluorescence microscopy was used to study two late endosomal proteins, Rab7 and LAMP1, that appear to be highly colocalized in static fluorescence microscopy images. Imaging these proteins simultaneously reveals that Rab7 and LAMP1 undergo periods of separation within the cell. Single particle tracking carried out during these periods of separation shows that Rab7-vesicles have greater velocities, but undergo less efficient transport than LAMP1-vesicles. This research demonstrates the use of single particle tracking as a tool to resolve functional differences in highly colocalized proteins in intact live cells.

Graphical abstract: Single particle tracking as a method to resolve differences in highly colocalized proteins

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Publication details

The article was received on 03 Nov 2010, accepted on 16 Dec 2010 and first published on 31 Jan 2011


Article type: Paper
DOI: 10.1039/C0AN00855A
Citation: Analyst, 2011,136, 3527-3533
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    Single particle tracking as a method to resolve differences in highly colocalized proteins

    C. J. Szymanski, W. H. Humphries, IV and C. K. Payne, Analyst, 2011, 136, 3527
    DOI: 10.1039/C0AN00855A

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