Issue 4, 2011
From the journal:

Analyst

Bisulfite-free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based flow cytometry assay†

Simon R. Corrie,ab   Pavel Sova,b   Qinghua Feng,b   Tiffany Blair,b   Nancy B. Kiviatb  and  Matt Trau*a  

* Corresponding authors

a The University of Queensland, Australian Institute for Bioengineering and Nanotechnology, Centre for Biomarker Research and Development, St Lucia, Brisbane, Australia
E-mail: m.trau@uq.edu.au
Fax: +61 7 3346 3973
Tel: +61 7 3346 4173

b University of Washington, School of Medicine, Department of Pathology, Seattle, USA

Abstract

DNA methylation analysis is emerging as a new technique with potential capabilities for early cancer detection. However, current state-of-the-art techniques are not easily translatable into routine clinical methods. Herein we describe a bead-based flow cytometry assay which combines DNA hybridization to microparticles with 5MeC-specific proteins/antibodies. These assays can be used to study the binding properties of current and emerging 5MeC-binding proteins and may also have potential in the measurement of 5MeC density in clinical samples for cancer detection.

Graphical abstract: Bisulfite-free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based flow cytometry assay

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Article information

DOI
https://doi.org/10.1039/C0AN00790K
Article type
Communication
Submitted
12 Oct 2010
Accepted
04 Nov 2010
First published
22 Nov 2010

Analyst, 2011,136, 688-691

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Bisulfite-free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based flow cytometry assay

S. R. Corrie, P. Sova, Q. Feng, T. Blair, N. B. Kiviat and M. Trau, Analyst, 2011, 136, 688 DOI: 10.1039/C0AN00790K

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