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Issue 18, 2011
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Zinc-finger motif noncovalent interactions with double-stranded DNA characterized by negative-ion electrospray ionization mass spectrometry

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Abstract

A zinc-finger motif recognizes specific sequences on the double helical structure of DNA. This sequence recognition property offers great promise for various biotechnology applications. Accordingly, it is crucially important to characterize zinc-finger binding characteristics for further developments. Although the gel shift assay or phage display is traditionally used for determining the binding characteristics of zinc-fingers for double stranded DNA, in the present study we utilize electrospray ionization mass spectrometry as an advanced and convenient characterization tool because of the rich information it provides, and its quantitative sensitivity, operational simplicity, and no need for radioactive labeling. Here we demonstrate the use of negative-ion electrospray ionization mass spectrometry for competition-based quantitative comparison of the zinc-finger motif sequence specificity, stoichiometry, and metal ion dependence.

Graphical abstract: Zinc-finger motif noncovalent interactions with double-stranded DNA characterized by negative-ion electrospray ionization mass spectrometry

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Publication details

The article was received on 03 May 2011, accepted on 23 Jun 2011 and first published on 21 Jul 2011


Article type: Paper
DOI: 10.1039/C1AN15376E
Citation: Analyst, 2011,136, 3739-3746
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    Zinc-finger motif noncovalent interactions with double-stranded DNA characterized by negative-ion electrospray ionization mass spectrometry

    S. Park, K. Jo and H. B. Oh, Analyst, 2011, 136, 3739
    DOI: 10.1039/C1AN15376E

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