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Issue 16, 2011
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Label-free fluorescent detection of thrombin using G-quadruplex-based DNAzyme as sensing platform

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Abstract

We report herein a label-free and sensitive fluorescent method for detection of thrombin using a G-quadruplex-based DNAzyme as the sensing platform. The thrombin-binding aptamer (TBA) is able to bind hemin to form the G-quadruplex-based DNAzyme, and thrombin can significantly enhance the activity of the G-quadruplex-based DNAzyme. The G-quadruplex-based DNAzyme is found to effectively catalyze the H2O2-mediated oxidation of thiamine, giving rise to fluorescence emission. This allows us to utilize the H2O2thiamine fluorescent system for the quantitative analysis of thrombin. The assay shows a linear toward thrombin concentration in the range of 0.01–0.12 nM. The present limit of detection for thrombin is 1 pM, and the sensitivity for analyzing thrombin is improved by about 10 000-fold as compared with the reported colorimetric counterpart. The work also demonstrates that thiamine is an excellent substrate for the fluorescence assay using the G-quadruplex-based DNAzyme as the sensing platform.

Graphical abstract: Label-free fluorescent detection of thrombin using G-quadruplex-based DNAzyme as sensing platform

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Publication details

The article was received on 02 Jan 2011, accepted on 27 May 2011 and first published on 04 Jul 2011


Article type: Paper
DOI: 10.1039/C1AN00002K
Citation: Analyst, 2011,136, 3268-3273
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    Label-free fluorescent detection of thrombin using G-quadruplex-based DNAzyme as sensing platform

    Y. Zhang, B. Li and Y. Jin, Analyst, 2011, 136, 3268
    DOI: 10.1039/C1AN00002K

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