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Issue 17, 2011
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Multiplexed evaluation of capture agent binding kinetics using arrays of silicon photonic microring resonators

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Abstract

High affinity capture agents recognizing biomolecular targets are essential in the performance of many proteomic detection methods. Herein, we report the application of a label-free silicon photonic biomolecular analysis platform for simultaneously determining kinetic association and dissociation constants for two representative protein capture agents: a thrombin-binding DNA aptamer and an anti-thrombin monoclonal antibody. The scalability and inherent multiplexing capability of the technology make it an attractive platform for simultaneously evaluating the binding characteristics of multiple capture agents recognizing the same target antigen, and thus a tool complementary to emerging high-throughput capture agent generation strategies.

Graphical abstract: Multiplexed evaluation of capture agent binding kinetics using arrays of silicon photonic microring resonators

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Publication details

The article was received on 02 Nov 2010, accepted on 04 Nov 2010 and first published on 18 Nov 2010


Article type: Communication
DOI: 10.1039/C0AN00853B
Citation: Analyst, 2011,136, 3430-3433
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    Multiplexed evaluation of capture agent binding kinetics using arrays of silicon photonic microring resonators

    J. Byeon and R. C. Bailey, Analyst, 2011, 136, 3430
    DOI: 10.1039/C0AN00853B

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