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Issue 6, 2011
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Techniques for recording reconstituted ion channels

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This review describes and discusses techniques useful for monitoring the activity of protein ion channelsin vitro. In the first section the biological importance and the classification of ion channels are outlined in order to justify the strong motivation for dealing with this important class of membrane proteins. The expression, reconstitution and integration of recombinant proteins into lipid bilayers are crucial steps to obtain consistent data when working with ion channels. In the second section recording techniques used in research are presented. Since this review focuses on analytical systems bearing reconstituted ion channels the industrial most important patch-clamp techniques of cells are only briefly mentioned. In section three, artificial systems developed in the last decades are described while the emerging technologies using nanostructured supports or microfluidic systems are presented in section four. Finally, the remaining challenges of membrane protein analysis and its potential applications are briefly outlined.

Graphical abstract: Techniques for recording reconstituted ion channels

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Publication details

The article was received on 27 Oct 2010, accepted on 08 Dec 2010 and first published on 25 Jan 2011

Article type: Critical Review
DOI: 10.1039/C0AN00828A
Citation: Analyst, 2011,136, 1077-1089
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    Techniques for recording reconstituted ion channels

    S. Demarche, K. Sugihara, T. Zambelli, L. Tiefenauer and J. Vörös, Analyst, 2011, 136, 1077
    DOI: 10.1039/C0AN00828A

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