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Issue 4, 2011
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Study of tissue engineered bone nodules by Fourier transform infrared spectroscopy

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Abstract

The key criteria for assessing the success of bone tissue engineering are the quality and quantity of the produced minerals within the cultured constructs. The accumulation of calcium ions and inorganic phosphates in culture medium serves as nucleating agents for the formation of hydroxyapatite, which is the main inorganic component of bone. Bone nodule formation is one of the hallmarks of mineralization in such cell cultures. In this study, we developed a new two-step procedure to accelerate bone formation in which mouse bone cell aggregates were produced first on various chemically treated non-adhesive substrates. After this step, the bone cells' growth and mineralization were followed in conventional culture plates. The number and size of cell aggregates were studied with light microscopy. The minerals' formation in the form of nodules produced by the cell aggregates and the bone crystal quality were studied with Fourier Transform Infrared (FTIR) spectroscopy. The FTIR spectra of the ash specimens (mineral phase only) from thermal gravimetric analysis (TGA) provided valuable information of the quality of the minerals. The υ4PO4 region (550–650 cm−1), which reveals apatitic and non-apatitic HPO4 or PO4 environments, and phosphate region (910–1180 cm−1) were examined for the minerals produced in the form of nodules. The peak position and intensity of the spectra demonstrate that the quality of the bone produced by cell aggregates, especially from the bigger ones, which were formed on Plunoric treated substrates, exhibit a composition more similar to that of native bone. This work establishes a new protocol for high quality bone formation and characterization, with the potential to be applied to bone tissue engineering.

Graphical abstract: Study of tissue engineered bone nodules by Fourier transform infrared spectroscopy

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Publication details

The article was received on 16 Jul 2010, accepted on 17 Nov 2010 and first published on 13 Dec 2010


Article type: Paper
DOI: 10.1039/C0AN00530D
Citation: Analyst, 2011,136, 775-780
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    Study of tissue engineered bone nodules by Fourier transform infrared spectroscopy

    H. M. Aydin, B. Hu, J. Sulé Suso, A. El Haj and Y. Yang, Analyst, 2011, 136, 775
    DOI: 10.1039/C0AN00530D

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