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Issue 20, 2010
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Fibronectin-mimetic peptide-amphiphile nanofiber gels support increased cell adhesion and promote ECM production

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Abstract

The design of nanofiber scaffolds has been a key objective in tissue engineering as they structurally mimic the natural extracellular matrix (ECM) found in tissues. The continuous cross talk between cells and the surrounding matrix ligands plays a crucial role during development and wound healing which leads to tissue regeneration and repair. In an attempt to provide a nanofiber scaffold with a ligand that can promote cell adhesion and ECM production, we propose the use of our peptide-amphiphile nanofibers as a potential scaffold for tissue engineering. A fibronectin-mimetic peptide sequence containing both the cell-binding GRGDSP domain and the synergistic PHSRN domain has been recently developed in our group. It has been shown to be a specific ligand for the α5β1 integrin, and has been synthesized to a C16 single-tailed peptide-amphiphile, PR_g (with a peptide headgroup of GGGSSPHSRN(SG)5RGDSP). The PR_g peptide-amphiphiles self-assemble into nanofibers in an aqueous environment and form hydrogels. In this study, the PR_g hydrogels were characterized with cryo-scanning electron microscopy (cryo-SEM) and rheology measurements, and their surfaces were evaluated in terms of sustaining long term human umbilical vein endothelial cell (HUVEC) adhesion and proliferation, cytoskeleton formation, and secretion of extracellular fibronectin. PR_g gels were compared to polyethylene glycol (PEG) gels, PEG gels functionalized with fibronectin as well as commercially available peptide hydrogels (PuraMatrix). Our results show that the PR_g hydrogels outperform the other gels evaluated in this study and therefore hold promise as scaffolds for tissue engineering.

Graphical abstract: Fibronectin-mimetic peptide-amphiphile nanofiber gels support increased cell adhesion and promote ECM production

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Publication details

The article was received on 04 May 2010, accepted on 23 Jun 2010 and first published on 04 Aug 2010


Article type: Paper
DOI: 10.1039/C0SM00321B
Citation: Soft Matter, 2010,6, 5064-5072
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    Fibronectin-mimetic peptide-amphiphile nanofiber gels support increased cell adhesion and promote ECM production

    K. Shroff, E. L. Rexeisen, M. A. Arunagirinathan and E. Kokkoli, Soft Matter, 2010, 6, 5064
    DOI: 10.1039/C0SM00321B

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