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Issue 16, 2010
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Fluorescent S-layer protein colloids

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Abstract

Four different coloured pH dependent biocolloids have been designed with fluorescent S-layer fusion proteins. The intrinsic self assembling capability of the S-layer protein SgsE on silicon dioxide particles lead to crystalline p2 lattice symmetry which was combined with the fluorescent properties of fused cyan ECFP, green EGFP, yellow YFP and red mRFP1. Confocal microscopy was used to monitor the pH dependence of the fluorescent S-layer coating. Electrophoretic measurements were carried out to understand the colloidal behaviour of the S-layer protein coated particles. It was found with flow cytometry that the stable SgsE-ECFP, SgsE-EGFP, SgsE-YFP and SgsE-mRFP bioparticle suspensions lost 50% of their fluorescence emission at the pKa of the chromophores. These novel fluorescent S-layer biocolloids can be used as pH sensors and might have an important role in surface pH determination.

Graphical abstract: Fluorescent S-layer protein colloids

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Publication details

The article was received on 19 Feb 2010, accepted on 06 May 2010 and first published on 29 Jun 2010


Article type: Paper
DOI: 10.1039/C0SM00008F
Citation: Soft Matter, 2010,6, 3809-3814
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    Fluorescent S-layer protein colloids

    B. Kainz, K. Steiner, U. B. Sleytr, D. Pum and J. L. Toca-Herrera, Soft Matter, 2010, 6, 3809
    DOI: 10.1039/C0SM00008F

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