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Issue 17, 2010
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The dynamics, kinetics and reversibility of protein adsorption onto the surface of biodegradable materials

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Abstract

Dynamic contact angle (DCA) analysis was used to investigate the kinetics and dynamics of protein interactions in a time-dependent manner for a group of organic natural-based surfaces during their initial contact with aqueous and protein solutions. Starch-based biomaterials were used to analyze the influence different materials with different surfaces had on the adsorption, desorption and configuration of proteins. Polymeric blends of starch and cellulose acetate (SCA), polycaprolactone (SPCL) and ethylene vinyl alcohol (SEVA-C) were used. The model protein systems included single protein solutions of human serum albumin, fibronectin, vitronectin and fibrinogen, and also complex solutions of human blood plasma. In the adsorption studies, very small and nearly equal advancing and receding contact angles were measured for all the materials. Highly wetting and low contact angle hysteresis, therefore, are exhibited by these surfaces. This effect was more noticeable for SCA surfaces. Moreover, the effect of protein concentration was also assessed and demonstrated to substantially affect the DCA wetting forces of SEVA-C and SPCL surfaces. In the desorption studies, during the rinsing phase with saline solution, the DCA loops became larger than that observed for the adsorption phase, which indicated increases in the contact angle hysteresis. The hysteresis of SCA and SPCL surfaces reversibly changed through the desorption phase, at the end of which, hysteresis was comparable to that of surfaces immersed in saline solution. The results indicated that adsorbed proteins could desorb more readily on SCA and SPCL than on SEVA-C. In the later case, stronger interactions such as hydrophobic forces were established and it is likely the rearrangement of protein conformation had occurred. Monitored by DCA, the evolution of hysteresis demonstrated the progressive bond strengthening between protein molecules and solid substratum, further elucidating the behaviour of proteins that regulate cellular interactions with implanted devices.

Graphical abstract: The dynamics, kinetics and reversibility of protein adsorption onto the surface of biodegradable materials

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Publication details

The article was received on 11 Feb 2010, accepted on 10 May 2010 and first published on 30 Jul 2010


Article type: Paper
DOI: 10.1039/C002972F
Citation: Soft Matter, 2010,6, 4135-4143
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    The dynamics, kinetics and reversibility of protein adsorption onto the surface of biodegradable materials

    C. M. Alves, R. L. Reis and J. A. Hunt, Soft Matter, 2010, 6, 4135
    DOI: 10.1039/C002972F

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