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Issue 5, 2010
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Self-assembled charged hydrogels control the alignment of filamentous actin

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Abstract

We demonstrate a novel route to control attachment of filamentous actin (F-actin) on hydrogel films. By incorporating an amine-terminated silane, the hydrogel surface charge and surface topography are varied. With increasing silane content, F-actin reorients from perpendicular to parallel to the hydrogel surface, ceases to wobble, and forms mainly elongated or cyclic structures. F-Actin coverage reaches a maximum at 2.5 vol% silane and declines at higher silane content. This biphasic behavior is explained by the simultaneous increase in surface charge and the self-assembly of a micron scale pattern of positively charged islands. Our approach provides guidelines for constructing nanoscale tracks to guide motor proteins underlying nano-engineered devices such as molecular shuttles.

Graphical abstract: Self-assembled charged hydrogels control the alignment of filamentous actin

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Publication details

The article was received on 04 Sep 2009, accepted on 30 Nov 2009 and first published on 21 Dec 2009


Article type: Paper
DOI: 10.1039/B918304C
Citation: Soft Matter, 2010,6, 915-921
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    Self-assembled charged hydrogels control the alignment of filamentous actin

    J. H. Park, Y. Sun, Y. E. Goldman and R. J. Composto, Soft Matter, 2010, 6, 915
    DOI: 10.1039/B918304C

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