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Issue 1, 2010
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Definition of the uptake mechanism and sub-cellular localisation profile of emissive lanthanide complexes as cellular optical probes

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Abstract

A series of experiments has been undertaken in order to gain a greater understanding of the cellular uptake and localisation behaviour of emissive lanthanide complexes as cellular stains or probes. Out of a large number of structurally related complexes characterised recently, a set of seven representative examples has been examined in detail, containing either tetraazatriphenylene or azaxanthone-based sensitising chromophores. Intracellular localisation profiles and cellular uptake and egress behaviour have been studied by microscopy and flow cytometry. Typically, the maximum intracellular concentration was of the order of 0.4 mM, or about 109 complexes per cell. The complexes studied were generally not toxic and did not perturb the mitochondrial membrane potential. A common uptake mechanism of macropinocytosis has been identified. A generalisation of trends in behaviour, and structure–activity relationships is presented, and the implications for future probe design discussed.

Graphical abstract: Definition of the uptake mechanism and sub-cellular localisation profile of emissive lanthanide complexes as cellular optical probes

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Publication details

The article was received on 04 Jan 2010, accepted on 16 Mar 2010 and first published on 12 May 2010


Article type: Edge Article
DOI: 10.1039/C0SC00105H
Citation: Chem. Sci., 2010,1, 111-118
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    Definition of the uptake mechanism and sub-cellular localisation profile of emissive lanthanide complexes as cellular optical probes

    E. J. New, A. Congreve and D. Parker, Chem. Sci., 2010, 1, 111
    DOI: 10.1039/C0SC00105H

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