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Issue 6, 2010
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Membrane protein biosensing with plasmonic nanopore arrays and pore-spanning lipid membranes

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Abstract

Integration of solid-state biosensors and lipid bilayer membranes is important for membrane protein research and drug discovery. In these sensors, it is critical that the solid-state sensing material does not have adverse effects on the conformation or functionality of membrane-bound molecules. In this work, pore-spanning lipid membranes are formed over an array of periodic nanopores in free-standing gold films for surface plasmon resonance (SPR) kinetic binding assays. The ability to perform kinetic assays with a transmembrane protein is demonstrated with α-hemolysin (α-HL). The incorporation of α-HL into the membrane followed by specific antibody binding (anti-α-HL) red-shifts the plasmon resonance of the gold nanopore array, which is optically monitored in real time. Subsequent fluorescence imaging reveals that the antibodies primarily bind in nanopore regions, indicating that α-HL incorporation preferentially occurs into areas of pore-spanning lipid membranes.

Graphical abstract: Membrane protein biosensing with plasmonic nanopore arrays and pore-spanning lipid membranes

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Publication details

The article was received on 09 Jul 2010, accepted on 20 Sep 2010 and first published on 13 Oct 2010


Article type: Edge Article
DOI: 10.1039/C0SC00365D
Citation: Chem. Sci., 2010,1, 688-696
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    Membrane protein biosensing with plasmonic nanopore arrays and pore-spanning lipid membranes

    H. Im, N. J. Wittenberg, A. Lesuffleur, N. C. Lindquist and S. Oh, Chem. Sci., 2010, 1, 688
    DOI: 10.1039/C0SC00365D

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