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Issue 6, 2010
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Conjugation of RAFT-generated polymers to proteins by two consecutive thiolene reactions

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Abstract

Well-defined temperature-responsive polymers were covalently conjugated to model proteins by two consecutive Michael addition thiolene reactions. Poly(N-isopropylacrylamide) (PNIPAM) prepared by reversible addition–fragmentation chain transfer (RAFT) polymerization was aminolyzed to yield thiol-terminated chains that were subsequently reacted with excess 1,8-bis-maleimidodiethyleneglycol. The resulting maleimide-terminated polymer was reacted with bovine serum albumin and ovalbumin to yield polymerprotein conjugates by a “grafting-to” approach. The thermoresponsive nature of PNIPAM was conferred to the conjugate, as demonstrated by dynamic light scattering analysis that indicated the formation of intermolecular aggregates at elevated temperatures.

Graphical abstract: Conjugation of RAFT-generated polymers to proteins by two consecutive thiol–ene reactions

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Publication details

The article was received on 22 Jan 2010, accepted on 15 Feb 2010 and first published on 29 Mar 2010


Article type: Paper
DOI: 10.1039/C0PY00025F
Citation: Polym. Chem., 2010,1, 854-859
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    Conjugation of RAFT-generated polymers to proteins by two consecutive thiolene reactions

    M. Li, P. De, H. Li and B. S. Sumerlin, Polym. Chem., 2010, 1, 854
    DOI: 10.1039/C0PY00025F

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