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Issue 10, 2010
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A photoswitchable DNA-binding protein based on a truncated GCN4-photoactive yellow protein chimera

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Abstract

Photo-controlled DNA-binding proteins promise to be useful tools for probing complex spatiotemporal patterns of gene expression in living organisms. Here we report a novel photoswitchable DNA-binding protein, GCN4(S)Δ25PYP, based on a truncated GCN4-photoactive yellow protein chimera. In contrast to previously reported designed photoswitchable proteins where DNA binding affinity is enhanced upon irradiation, GCN4(S)Δ25PYP dissociates from DNA when irradiated with blue light. In addition, the rate of thermal relaxation to the ground state, part of the PYP photocycle, is enhanced by DNA binding whereas in previous reported constructs it is slowed. The origins of this reversed photoactivity are analyzed in structural terms.

Graphical abstract: A photoswitchable DNA-binding protein based on a truncated GCN4-photoactive yellow protein chimera

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Publication details

The article was received on 12 Jul 2010, accepted on 15 Aug 2010 and first published on 13 Sep 2010


Article type: Paper
DOI: 10.1039/C0PP00214C
Citation: Photochem. Photobiol. Sci., 2010,9, 1320-1326
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    A photoswitchable DNA-binding protein based on a truncated GCN4-photoactive yellow protein chimera

    S. Morgan and G. A. Woolley, Photochem. Photobiol. Sci., 2010, 9, 1320
    DOI: 10.1039/C0PP00214C

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