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Issue 5, 2010
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Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters

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Abstract

The fluorescence of silver clusters encapsulated by single stranded oligo-DNA (24 cytosine base pairs, C24:Agn) was used to monitor the transfection of this new silver/DNA fluorophore inside living HeLa cells. For this, the C24:Agn molecules were complexed with a commercially available transfection reagent Lipofectamine and the internalization of C24:Agn was followed with confocal fluorescence microscopy. Bright near-infrared fluorescence was observed from inside the transfected HeLa cells, when exciting with 633 nm excitation, opening up the possibility for the use of these C24:Agn clusters for biological labelling and imaging of living cells and for monitoring the transfection process with limited harm to the living cells.

Graphical abstract: Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters

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Publication details

The article was received on 03 Feb 2010, accepted on 05 Mar 2010 and first published on 22 Mar 2010


Article type: Paper
DOI: 10.1039/C0PP00015A
Citation: Photochem. Photobiol. Sci., 2010,9, 716-721
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    Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters

    Y. Antoku, J. Hotta, H. Mizuno, R. M. Dickson, J. Hofkens and T. Vosch, Photochem. Photobiol. Sci., 2010, 9, 716
    DOI: 10.1039/C0PP00015A

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