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Issue 23, 2010
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Polymeric PARACEST MRI contrast agents as potential reporters for gene therapy

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Abstract

Gene therapy is a potentially powerful treatment approach that targets molecular remedies for disease. Among other challenges it remains difficult to monitor gene delivery and its downstream metabolic consequences. Approaches to MRI gene reporters have been reported but few have the potential for translation beyond isolated cell systems. Herein, we report a polycationic polymer MRI contrast agent that binds to DNA in a ratio of one monomer unit per phosphate group of DNA. Significantly, this binding event diminishes the MR contrast signal from the agent itself potentially providing a platform for imaging delivery and release of a gene into cells and tissues. Importantly, we demonstrate here the proof of concept that a positively charged polymeric contrast agent can also act as a transfection agent, delivering the gene for encoding green fluorescent protein into cells. These observations provide support for the radical, new idea of creating a combined transfection/imaging agent for monitoring gene delivery in real time by MRI.

Graphical abstract: Polymeric PARACEST MRI contrast agents as potential reporters for gene therapy

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Publication details

The article was received on 06 May 2010, accepted on 09 Aug 2010 and first published on 16 Sep 2010


Article type: Paper
DOI: 10.1039/C0OB00087F
Citation: Org. Biomol. Chem., 2010,8, 5333-5338
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    Polymeric PARACEST MRI contrast agents as potential reporters for gene therapy

    Y. Wu, C. E. Carney, M. Denton, E. Hart, P. Zhao, D. N. Streblow, A. D. Sherry and M. Woods, Org. Biomol. Chem., 2010, 8, 5333
    DOI: 10.1039/C0OB00087F

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