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Issue 14, 2010
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The Glc2Man2-fragment of the N-glycan precursor – a novel ligand for the glycan-binding protein malectin?

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Abstract

The Glcα(1→3)Glcα(1→3)Manα(1→2)Man tetrasaccharide (Glc2Man2-fragment), a substructure of the natural N-glycan precursor, was synthesized. The interaction of this fragment with the protein malectin, a carbohydrate binding protein localized in the endoplasmatic reticulum, was investigated by 1H15N HSQC experiments and isothermal calorimetry. The chemical shift perturbations of nuclei in the protein's backbone caused by the binding of the Glc2Man2-fragment to malectin suggest a binding mode like the known ligand nigerose.

Graphical abstract: The Glc2Man2-fragment of the N-glycan precursor – a novel ligand for the glycan-binding protein malectin?

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Publication details

The article was received on 19 Mar 2010, accepted on 13 May 2010 and first published on 26 May 2010


Article type: Paper
DOI: 10.1039/C004502K
Citation: Org. Biomol. Chem., 2010,8, 3294-3299
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    The Glc2Man2-fragment of the N-glycan precursor – a novel ligand for the glycan-binding protein malectin?

    L. N. Müller, C. Muhle-Goll and M. B. Biskup, Org. Biomol. Chem., 2010, 8, 3294
    DOI: 10.1039/C004502K

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