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Issue 6, 2010
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Structural mass spectrometry analysis of lipid changes in a Drosophila epilepsy model brain

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Abstract

Phosphatidylethanolamine (PtdEtn) is one of the most abundant phospholipids in many animal cell types. The Drosophila easily shocked (eas2) mutant, used as an epilepsy model, is null for the PtdEtn biosynthetic enzyme, ethanolamine kinase. This mutant displays bang sensitive paralysis, and was previously shown to have decreased levels of PtdEtn. We have developed a highly selective and sensitive measurement strategy using ion mobility-mass spectrometry for the relative quantitation of intact phospholipid species directly from isolated brain tissue of eas mutants. Over 1200 distinct lipid signals are observed and within this population 38, including PtdEtn, phosphatidylinositol (PtdIns) and phosphatidylcholine (PtdCho) species are identified to have changed significantly (p < 0.03) between mutant and control tissue. This method has revealed for the first time the structural complexity and biosynthetic interconnectedness of specific PtdEtn and PtdIns lipid species within tissue, and provides great molecular detail compared to traditionally used detection techniques.

Graphical abstract: Structural mass spectrometry analysis of lipid changes in a Drosophila epilepsy model brain

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Publication details

The article was received on 05 Jan 2010, accepted on 12 Feb 2010 and first published on 09 Apr 2010


Article type: Paper
DOI: 10.1039/B927494D
Citation: Mol. BioSyst., 2010,6, 958-966
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    Structural mass spectrometry analysis of lipid changes in a Drosophila epilepsy model brain

    M. Kliman, N. Vijayakrishnan, L. Wang, J. T. Tapp, K. Broadie and J. A. McLean, Mol. BioSyst., 2010, 6, 958
    DOI: 10.1039/B927494D

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