Jump to main content
Jump to site search

Issue 4, 2010
Previous Article Next Article

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Author affiliations

Abstract

By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three Nε-acetyl-L-lysines into one GFPUV protein in E. coli.

Graphical abstract: A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 Sep 2009, accepted on 26 Jan 2010 and first published on 15 Feb 2010


Article type: Communication
DOI: 10.1039/B920120C
Citation: Mol. BioSyst., 2010,6, 683-686
  •   Request permissions

    A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli

    Y. Huang, W. K. Russell, W. Wan, P. Pai, D. H. Russell and W. Liu, Mol. BioSyst., 2010, 6, 683
    DOI: 10.1039/B920120C

Search articles by author

Spotlight

Advertisements