Jump to main content
Jump to site search

Issue 1, 2010
Previous Article Next Article

In vivo proton HR-MAS NMR metabolic profile of the freshwater cladoceran Daphnia magna

Author affiliations

Abstract

The method concerning in vivo proton HR-MAS NMR metabolic profiling of the freshwater cladoceran Daphnia magna is presented. Viability tests of D. magna under different spinning rates were performed. All surviving daphnids after analysis have developed eggs and embryos like control animals. Better survival rate at the slowest rotation speed were observed. The maximum length of analysis during which the integrity of the daphnid is maintained was assessed. The recorded proton spectra of in vivo daphnia were attributed to lipids from the triglycerol category. Saturated and unsaturated omega-3 like fatty acid moieties of triacylglycerol were well identified. The relationship between physiological state of daphnids and lipid profile are discussed.

Graphical abstract: In vivo proton HR-MAS NMR metabolic profile of the freshwater cladoceran Daphnia magna

Back to tab navigation

Publication details

The article was received on 29 Jul 2009, accepted on 10 Sep 2009 and first published on 29 Sep 2009


Article type: Method
DOI: 10.1039/B915417E
Citation: Mol. BioSyst., 2010,6, 121-125
  •   Request permissions

    In vivo proton HR-MAS NMR metabolic profile of the freshwater cladoceran Daphnia magna

    A. Bunescu, J. Garric, B. Vollat, E. Canet-Soulas, D. Graveron-Demilly and F. Fauvelle, Mol. BioSyst., 2010, 6, 121
    DOI: 10.1039/B915417E

Search articles by author

Spotlight

Advertisements