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Issue 21, 2010
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Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR

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Abstract

An agarose droplet method was developed for highly parallel and efficient single molecule emulsion PCR. The method capitalizes on the unique thermoresponsive sol–gel switching property of agarose for highly efficient DNA amplification and amplicon trapping. Uniform agarose solution droplets generated via a microfluidic chip serve as robust and inert nanolitre PCR reactors for single copy DNA molecule amplification. After PCR, agarose droplets are gelated to form agarose beads, trapping all amplicons in each reactor to maintain the monoclonality of each droplet. This method does not require cocapsulation of primer labeled microbeads, allows high throughput generation of uniform droplets and enables high PCR efficiency, making it a promising platform for many single copy genetic studies.

Graphical abstract: Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR

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Publication details

The article was received on 24 Jun 2010, accepted on 09 Aug 2010 and first published on 13 Sep 2010


Article type: Communication
DOI: 10.1039/C0LC00145G
Citation: Lab Chip, 2010,10, 2841-2843
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    Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR

    X. Leng, W. Zhang, C. Wang, L. Cui and C. J. Yang, Lab Chip, 2010, 10, 2841
    DOI: 10.1039/C0LC00145G

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