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Issue 24, 2010
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Perfusion-based microfluidic device for three-dimensional dynamic primary human hepatocyte cell culture in the absence of biological or synthetic matrices or coagulants

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Abstract

We describe a perfusion-based microfluidic device for three-dimensional (3D) dynamic primary human hepatocyte cell culture. The microfluidic device was used to promote and maintain 3D tissue-like cellular morphology and cell-specific functionality of primary human hepatocytes by restoring membrane polarity and hepatocyte transport function in vitro without the addition of biological or synthetic matrices or coagulants. A unique feature of our dynamic cell culture device is the creation of a microenvironment, without the addition of biological or synthetic matrices or coagulants, that promotes the 3D organization of hepatocytes into cord-like structures that exhibit functional membrane polarity as evidenced by the expression of gap junctions and the formation of an extended, functionally active, bile canalicular network.

Graphical abstract: Perfusion-based microfluidic device for three-dimensional dynamic primary human hepatocyte cell culture in the absence of biological or synthetic matrices or coagulants

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Publication details

The article was received on 21 Jun 2010, accepted on 15 Oct 2010 and first published on 08 Nov 2010


Article type: Paper
DOI: 10.1039/C0LC00135J
Citation: Lab Chip, 2010,10, 3380-3386
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    Perfusion-based microfluidic device for three-dimensional dynamic primary human hepatocyte cell culture in the absence of biological or synthetic matrices or coagulants

    V. N. Goral, Y. Hsieh, O. N. Petzold, J. S. Clark, P. K. Yuen and R. A. Faris, Lab Chip, 2010, 10, 3380
    DOI: 10.1039/C0LC00135J

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