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Issue 22, 2010
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A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole blood

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Abstract

We describe an automated, self-powered chip based on lateral flow immunoassay for rapid, quantitative, and multiplex protein detection from pinpricks of whole blood. The device incorporates on-chip purification of blood plasma by employing inertial forces to focus blood cells away from the assay surface, where plasma proteins are captured and detected on antibody “barcode” arrays. Power is supplied from the capillary action of a piece of adsorbent paper, and sequentially drives, over a 40 minute period, the four steps required to capture serum proteins and then develop a multiplex immunoassay. An 11 protein panel is assayed from whole blood, with high sensitivity and high reproducibility. This inexpensive, self-contained, and easy to operate chip provides a useful platform for point-of-care diagnoses, particularly in resource-limited settings.

Graphical abstract: A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole blood

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Publication details

The article was received on 19 Jun 2010, accepted on 09 Sep 2010 and first published on 06 Oct 2010


Article type: Paper
DOI: 10.1039/C0LC00132E
Citation: Lab Chip, 2010,10, 3157-3162
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    A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole blood

    J. Wang, H. Ahmad, C. Ma, Q. Shi, O. Vermesh, U. Vermesh and J. Heath, Lab Chip, 2010, 10, 3157
    DOI: 10.1039/C0LC00132E

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