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Issue 21, 2010
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Uniform cell seeding and generation of overlapping gradient profiles in a multiplexed microchamber device with normally-closed valves

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Abstract

Generation of stable soluble-factor gradients in microfluidic devices enables studies of various cellular events such as chemotaxis and differentiation. However, many gradient devices directly expose cells to constant fluid flow and that can induce undesired responses from cells due to shear stress and/or wash out of cell-secreted molecules. Although there have been devices with flow-free gradients, they typically generate only a single condition and/or have a decaying gradient profile that does not accommodate long-term experiments. Here we describe a microdevice that generates several chemical gradient conditions on a single platform in flow-free microchambers which facilitates steady-state gradient profiles. The device contains embedded normally-closed valves that enable fast and uniform seeding of cells to all microchambers simultaneously. A network of microchannels distributes desired solutions from easy-access open reservoirs to a single output port, enabling a simple setup for inducing flow in the device. Embedded porous filters, sandwiched between the microchannel networks and cell microchambers, enable diffusion of biomolecules but inhibit any bulk flow over the cells.

Graphical abstract: Uniform cell seeding and generation of overlapping gradient profiles in a multiplexed microchamber device with normally-closed valves

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Publication details

The article was received on 09 Jun 2010, accepted on 17 Aug 2010 and first published on 09 Sep 2010


Article type: Paper
DOI: 10.1039/C0LC00086H
Citation: Lab Chip, 2010,10, 2959-2964
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    Uniform cell seeding and generation of overlapping gradient profiles in a multiplexed microchamber device with normally-closed valves

    B. Mosadegh, M. Agarwal, H. Tavana, T. Bersano-Begey, Y. Torisawa, M. Morell, M. J. Wyatt, K. S. O'Shea, K. F. Barald and S. Takayama, Lab Chip, 2010, 10, 2959
    DOI: 10.1039/C0LC00086H

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