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Issue 19, 2010
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Micropillar array chips toward new immunodiagnosis

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Abstract

In this paper, we demonstrate the possibility to use a micropillar array to perform molecular immunodiagnosis. A polydimethylsiloxane (PDMS) microdevice consisting of a rectangular array of micropillars (45 μm in height, 100 × 100 μm square cross section) was used to replace microchannels or gels (polyacrylamide or agarose) to perform electrokinetic separation. This microarray was used to mimic highly diluted gel and to maintain electrolyte within the pillar zone by capillary effect. The electrolyte composition (glycerol and agarose content) was investigated in order to improve protein separation by isoelectric focusing (IEF). The influence of glycerol on focusing time and on the different evaporative contributions was further evaluated. In order to perform an immunodiagnostic of milk allergy, different surface treatments were optimized to prevent milk allergen adsorption on PDMS surface. Poly(dimethylacrylamide)-co-allyl glycidyl ether (PDMA-AGE) as well as gelatin led to a satisfactory signal to noise ratio. Finally the possibility to perform protein mixture separation using this micropillar array chip followed by immunoblotting was demonstrated by using the serum from an allergic individual, confirming the great potential of this analytical platform in the field of immunodiagnosis.

Graphical abstract: Micropillar array chips toward new immunodiagnosis

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Publication details

The article was received on 12 Apr 2010, accepted on 14 Jun 2010 and first published on 16 Aug 2010


Article type: Paper
DOI: 10.1039/C005034B
Citation: Lab Chip, 2010,10, 2597-2604
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    Micropillar array chips toward new immunodiagnosis

    H. Li, V. Dauriac, V. Thibert, H. Senechal, G. Peltre, X. Zhang and S. Descroix, Lab Chip, 2010, 10, 2597
    DOI: 10.1039/C005034B

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