Jump to main content
Jump to site search

Issue 20, 2010
Previous Article Next Article

An agar gel membrane-PDMS hybrid microfluidic device for long term single cell dynamic study

Author affiliations

Abstract

Significance of single cell measurements stems from the substantial temporal fluctuations and cell-cell variability possessed by individual cells. A major difficulty in monitoring surface non-adherent cells such as bacteria and yeast is that these cells tend to aggregate into clumps during growth, obstructing the tracking or identification of single-cells over long time periods. Here, we developed a microfluidic platform for long term single-cell tracking and cultivation with continuous media refreshing and dynamic chemical perturbation capability. The design highlights a simple device-assembly process between PDMS microchannel and agar membrane through conformal contact, and can be easily adapted by microbiologists for their routine laboratory use. The device confines cell growth in monolayer between an agar membrane and a glass surface. Efficient nutrient diffusion through the membrane and reliable temperature maintenance provide optimal growth condition for the cells, which exhibited fast exponential growth and constant distribution of cell sizes. More than 24 h of single-cell tracking was demonstrated on a transcription-metabolism integrated synthetic biological model, the gene-metabolic oscillator. Single cell morphology study under alcohol toxicity allowed us to discover and characterize cell filamentation exhibited by different E. coli isobutanol tolerant strains. We believe this novel device will bring new capabilities to quantitative microbiology, providing a versatile platform for single cell dynamic studies.

Graphical abstract: An agar gel membrane-PDMS hybrid microfluidic device for long term single cell dynamic study

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 Mar 2010, accepted on 18 Jun 2010 and first published on 27 Jul 2010


Article type: Paper
DOI: 10.1039/C004719H
Citation: Lab Chip, 2010,10, 2710-2719
  •   Request permissions

    An agar gel membrane-PDMS hybrid microfluidic device for long term single cell dynamic study

    I. Wong, S. Atsumi, W. Huang, T. Wu, T. Hanai, M. Lam, P. Tang, J. Yang, J. C. Liao and C. Ho, Lab Chip, 2010, 10, 2710
    DOI: 10.1039/C004719H

Search articles by author

Spotlight

Advertisements