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Issue 20, 2010
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Ultrasound-controlled cell aggregation in a multi-well chip

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We demonstrate a microplate platform for parallelized manipulation of particles or cells by frequency-modulated ultrasound. The device, consisting of a silicon-glass microchip and a single ultrasonic transducer, enables aggregation, positioning and high-resolution microscopy of cells distributed in an array of 100 microwells centered on the microchip. We characterize the system in terms of temperature control, aggregation and positioning efficiency, and cell viability. We use time-lapse imaging to show that cells continuously exposed to ultrasound are able to divide and remain viable for at least 12 hours inside the device. Thus, the device can be used to induce and maintain aggregation in a parallelized fashion, facilitating long-term microscopy studies of, e.g., cellcell interactions.

Graphical abstract: Ultrasound-controlled cell aggregation in a multi-well chip

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Publication details

The article was received on 30 Mar 2010, accepted on 30 Jul 2010 and first published on 31 Aug 2010

Article type: Paper
DOI: 10.1039/C004707D
Citation: Lab Chip, 2010,10, 2727-2732
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    Ultrasound-controlled cell aggregation in a multi-well chip

    B. Vanherberghen, O. Manneberg, A. Christakou, T. Frisk, M. Ohlin, H. M. Hertz, B. Önfelt and M. Wiklund, Lab Chip, 2010, 10, 2727
    DOI: 10.1039/C004707D

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