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Issue 16, 2010
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Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapsulation

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Abstract

Cell-encapsulated hydrogels with complex three-dimensional (3D) structures were fabricated from photopolymerizable poly(ethylene glycol) diacrylate (PEGDA) using modified ‘top-down’ and ‘bottoms-up’ versions of a commercially available stereolithography apparatus (SLA). Swelling and mechanical properties were measured for PEGDA hydrogels with molecular weights (Mw) ranging from 700 to 10 000 Daltons (Da). Long-term viability of encapsulated NIH/3T3 cells was quantitatively evaluated using an MTS assay and shown to improve over 14 days by increasing the Mw of the hydrogels. Addition of adhesive RGDS peptide sequences resulted in increased cell viability, proliferation, and spreading compared to pristine PEG hydrogels of the same Mw. Spatial 3D layer-by-layer cell patterning was successfully demonstrated, and the feasibility of depositing multiple cell types and material compositions into distinct layers was established.

Graphical abstract: Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapsulation

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Publication details

The article was received on 16 Mar 2010, accepted on 11 Jun 2010 and first published on 05 Jul 2010


Article type: Paper
DOI: 10.1039/C004285D
Citation: Lab Chip, 2010,10, 2062-2070
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    Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapsulation

    V. Chan, P. Zorlutuna, J. H. Jeong, H. Kong and R. Bashir, Lab Chip, 2010, 10, 2062
    DOI: 10.1039/C004285D

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