Jump to main content
Jump to site search

Issue 15, 2010
Previous Article Next Article

Continuous two-phase flow miniaturised bioreactor for monitoring anaerobic biocatalysis by pentaerythritol tetranitrate reductase

Author affiliations

Abstract

A novel continuous recirculating two-phase flow miniaturised bioreactor was developed for biocatalytic transformations with the enzyme pentaerythritol tetranitrate reductase using on-chip spectroscopic detection of the organic and aqueous phases. A phase separation technique is described that uses electrostatic attraction to force charged droplets to merge back into the aqueous phase and thus allow the monitoring of both reaction phases during enzymatic turnover. We report an increased rate of enzyme catalysed reduction of trans-2-(2-nitrovinyl)thiophene, which was used as a model system to demonstrate the principles of the bioreactor design, compared to conventional macroscale experiments. Additional data obtained with ketoisophorone, trans-cinnamaldehyde and 2-methylmaleimide support our findings and provide a basis for improving the chemistry of biocatalysis.

Graphical abstract: Continuous two-phase flow miniaturised bioreactor for monitoring anaerobic biocatalysis by pentaerythritol tetranitrate reductase

Back to tab navigation

Supplementary files

Publication details

The article was received on 24 Feb 2010, accepted on 13 May 2010 and first published on 07 Jun 2010


Article type: Paper
DOI: 10.1039/C003561K
Citation: Lab Chip, 2010,10, 1929-1936
  •   Request permissions

    Continuous two-phase flow miniaturised bioreactor for monitoring anaerobic biocatalysis by pentaerythritol tetranitrate reductase

    S. Mohr, K. Fisher, N. S. Scrutton, N. J. Goddard and P. R. Fielden, Lab Chip, 2010, 10, 1929
    DOI: 10.1039/C003561K

Search articles by author

Spotlight

Advertisements