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Issue 14, 2010
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A microfluidic device for continuous cancer cell culture and passage with hydrodynamic forces

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Abstract

We demonstrate a novel and robust microfluidic chip with combined functions of continuous culture and output of PC-3 prostate cancer cells. With digital controls, polydimethylsiloxane (PDMS) flexible diaphragms are able to apply hydrodynamic shear forces on cultures, detaching a fraction of attached cancer cells from the surface for output while leaving others for reuse in subsequent cultures. The fractions of detached cells and remaining cells can be precisely controlled. The system has not only the advantages of small size, high cell culture efficiency, and digital control, but also of simple fabrication at low cost, easy operation and robust performance. The chip performs 9 passages during 30 days of continuous culture and shows promise as a durable design suitable for long-term cell output.

Graphical abstract: A microfluidic device for continuous cancer cell culture and passage with hydrodynamic forces

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Publication details

The article was received on 23 Feb 2010, accepted on 08 Apr 2010 and first published on 27 Apr 2010


Article type: Paper
DOI: 10.1039/C003509B
Citation: Lab Chip, 2010,10, 1807-1813
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    A microfluidic device for continuous cancer cell culture and passage with hydrodynamic forces

    L. Liu, K. Loutherback, D. Liao, D. Yeater, G. Lambert, A. Estévez-Torres, J. C. Sturm, R. H. Getzenberg and R. H. Austin, Lab Chip, 2010, 10, 1807
    DOI: 10.1039/C003509B

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