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Issue 10, 2010
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Microbead-based rolling circle amplification in a microchip for sensitive DNA detection

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Abstract

The sensitive detection and quantification of DNA targets in the food industry and in environmental and clinical settings are issues of utmost importance in ensuring contamination-free food, monitoring the environment, and battling disease. Selective probes coupled with powerful amplification techniques are therefore of major interest. In this study, we set out to create an integrated microchemical chip that benefits from microfluidic chip technology in terms of sensitivity and a strong detection methodology provided jointly by padlock probes and rolling circle amplification (RCA). Here, we have integrated padlock probes and RCA into a microchip. The chip uses solid phase capture in a microchannel to enable washing cycles and decrease analytical area, and employs on-bead RCA for single-molecule amplification and detection. We investigated the effects of reagent concentration and amount of padlock probes, and demonstrated the feasibility of detecting Salmonella.

Graphical abstract: Microbead-based rolling circle amplification in a microchip for sensitive DNA detection

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Publication details

The article was received on 05 Jan 2010, accepted on 22 Feb 2010 and first published on 12 Mar 2010


Article type: Paper
DOI: 10.1039/B927460J
Citation: Lab Chip, 2010,10, 1262-1266
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    Microbead-based rolling circle amplification in a microchip for sensitive DNA detection

    K. Sato, A. Tachihara, B. Renberg, K. Mawatari, K. Sato, Y. Tanaka, J. Jarvius, M. Nilsson and T. Kitamori, Lab Chip, 2010, 10, 1262
    DOI: 10.1039/B927460J

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