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Issue 7, 2010
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A rapid field-use assay for mismatch number and location of hybridized DNAs

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Abstract

Molecular dielectrophoresis (DEP) is employed to rapidly (<ms) trap ssDNA molecules in a flowing solution to a cusp-shaped nanocolloid assembly on a chip with a locally amplified AC electric field gradient. By tuning AC field frequency and DNA DEP mobility relative to its electrophoretic mobility due to electrostatic repulsion from like-charged nanocolloids, mismatch-specific binding of DNA molecules at the cusp is achieved by the converging flow, with a concentration factor about 6 orders of magnitude higher than the bulk, thus allowing fluorescent quantification of concentrated DNAs at the singularity in a generic buffer, at room temperature within a minute. Optimum flow rate and the corresponding hybridization rate change by nearly a factor of 2 with a single mismatch in the 26 base docking sequence and are also sensitive to the mismatch location. This dielectrophoresis and shear enhanced pico-molar sensitivity and SNP selectivity can hence be used for field-use DNA detection/identification.

Graphical abstract: A rapid field-use assay for mismatch number and location of hybridized DNAs

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Publication details

The article was received on 09 Dec 2009, accepted on 01 Feb 2010 and first published on 23 Feb 2010


Article type: Communication
DOI: 10.1039/B925854J
Citation: Lab Chip, 2010,10, 828-831
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    A rapid field-use assay for mismatch number and location of hybridized DNAs

    I. Cheng, S. Senapati, X. Cheng, S. Basuray, H. Chang and H. Chang, Lab Chip, 2010, 10, 828
    DOI: 10.1039/B925854J

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