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Issue 7, 2010
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High-throughput fluorescence detection using an integrated zone-plate array

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Abstract

Microfluidic devices enable massive parallelization of sample manipulation and delivery, but a similarly parallelized and integrated optical detection system does not yet exist. Standard large numerical aperture wide field or scanning optical systems are not capable of the large field of view and detection sensitivity required to collect fluorescence from parallel arrays of microfluidic devices. Instead, we present a fluorescence measurement platform based on a microfabricated zone-plate array integrated into a parallelized microfluidic device. The zone-plate array is orientated so that a single high numerical aperture zone plate is aligned to read out the fluorescence from each of 64 output channels of a drop-making device. The parallelization of microfluidics and optics produces an integrated system capable of analysis of nearly 200 000 drops per second.

Graphical abstract: High-throughput fluorescence detection using an integrated zone-plate array

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Publication details

The article was received on 10 Nov 2009, accepted on 16 Dec 2009 and first published on 18 Jan 2010


Article type: Paper
DOI: 10.1039/B923554J
Citation: Lab Chip, 2010,10, 852-856
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    High-throughput fluorescence detection using an integrated zone-plate array

    E. Schonbrun, A. R. Abate, P. E. Steinvurzel, David. A. Weitz and K. B. Crozier, Lab Chip, 2010, 10, 852
    DOI: 10.1039/B923554J

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